Key features and details | |
Cat. No. | MABL-1765 |
Name | Anti-H4 K8Ac; K12Ac; K16Ac mAbs |
Clone No. | AFD- KM-2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, ELISA, IF, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | KM-2 antibody recognizes the N-terminal peptides 1-20 of histone H2A and 1-29 of histone H4 which have a large sequence homology (van Bruggen et al., 1997; pmid: 9027774). Histone H4 acetylation on lysines 8, 12 and 16 significantly increases the antibody's binding while the acetylation at K5 decreases it (Dieker et al., 2007; pmid: 17530637). |
Alternative Name | histone 4; nucleosome; H4K8Ac; H4K12Ac; H4K16Ac; acetyl-Histone H4; Histone H4 acetylated at lysine; KM2 |
UniProt | |
Immunogen | KM-2 antibody was derived from an MRL/lpr lupus mouse. |
Application Notes | The antibody KM-2 shows significant increase in activity with histone H4 when it is acethylated on lysines 8, 12 and 16 which are hyperacethylated in conditions, such as lupus (SLE); and thus KM-2 can be used in the detection of the apoptosis-induced histone modifications characteristic of lupus and in the analysis of its pathogenesis (Dieker et al., 2007; pmid: 17530637). This antibody was successfully used to identify both Histone H4 as well as H2A in the extracts of Jurkat cells via Western blot, showing considerable increase in activity in cells with induced apoptosis leading to hyperacethylation (Dieker et al., 2007; pmid: 17530637). Similar results were achieved for the immunofluorescence staining of Jurkat cells. The enhanced reactivity of KM‐2 with apoptotic H4 and apoptotic H2A was not Jurkat cell specific and was also observed for the monocytic U937 cell line which indicated its general applicability (Dieker et al., 2007; pmid: 17530637). |
Antibody First Published | van Bruggen et al. Nucleosomes and histones are present in glomerular deposits in human lupus nephritis. Nephrol Dial Transplant. 1997 Jan;12(1):57-66. PMID:9027774 |
Note on publication | This article describes the characterization of the KM-2 (KM2) antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
