Anti-Unknown Specificity mAbs

Cat. No.

MABL-3173

Name

Anti-Unknown Specificity mAbs

Clone No.

AFD- NISTmab (RM 8671)

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Species Reactivity

Basic Information

Specificity

Alternative Name

mAb 8671; RM8671; NIST8671; NISTmab1;   reference material 8671

UniProt

Immunogen

Application Notes

This antibody is a recombinant version of NISTmAb, a humanized IgG1k reference material 8671 (RM 8671) from the National Institute of Standards and Technology. It is a widely characterized monoclonal antibody, intended for use in evaluating the performance of methods for determining physicochemical and biophysical attributes of monoclonal antibodies. This antibody acts as a representative test molecule for development of novel technology for therapeutic protein characterization. It is intended for a variety of uses that may include system suitability tests, establishing method or instrument performance and variability, comparing changing analytical test methods, and assisting in method qualification (Reference Material 8671 information sheet). This antibody can be used as a standard for the benchmarking of top-down mass spectrometry for   analysis of intact proteins (PMID: 31249407). It was also used as a standard for de novo sequencing algorithm on bottom-up mass spectrometry (PMID: 28105549). It was also used as a standard for calibration of a technique for  estimating the monomeric purity and antigen affinity of research grade   antibody samples (PMID: 31375854). 

Antibody First   Published

Note on publication

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.