Key features and details | |
Cat. No. | MABL-3174 |
Name | Anti-Urease alpha subunit mAbs |
Clone No. | AFD- HpU-2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | inhibit, SPR, ELISA |
Species Reactivity | Helicobacter pylori |
Basic Information | |
Specificity | HpU-2 binds specifically to H. pylori at the epitope with sequence SVELIDIGGNRRIFGFNALVDR - the sequence is part of a loop near to the C-terminus of the alpha subunit of urease (the beta subunit possesses the active site). HpU-18 competes with HpU-2 for binding to this epitope. H. pylori is a gram negative spiral bacterium and causes numerous gastroduodenal diseases. It expresses large amounts of urease when present in the human stomach; urease is key for the survival and pathogenesis of the bacteria as it neutralizes the acidity in the stomach by hydrolysis of urea into ammonia. |
Alternative Name | urease-alpha; alpha-urease; urease |
UniProt | P14916 |
Immunogen | HpU-2 was prepared by subcutaneous injection of purified H. pylori urease into BALB/c mice. |
Application Notes | HpU-2 results in ~82% inhibition (allosteric inhibition) of urease activity and the inhibitory activity is increased to 87% when HpU-2 is used in combination with HpU-19. HpU-2 has been shown be unsuccessful for use in IHC of formalin-fixed sections of H. pylori-infected human cells. HpU-2 can also be used in SPR and ELISA studies. |
Antibody First Published | Fujii et al. Epitope mapping and features of the epitope for monoclonal antibodies inhibiting enzymatic activity of Helicobacter pylori urease. Biotechnol Bioeng. 2004 May 20;86(4):434-44. PMID:15112296 |
Note on publication | Describes the investigation into the inhibitory mechanism of HpU-2 against H. pylori urease. The binding epitope was determined by SPR and molecular modelling. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
